phospho glur1 ser831  (Bioss)

Journal: eLife

Article Title: Ketamine’s rapid antidepressant effects are mediated by Ca 2+ -permeable AMPA receptors

doi: 10.7554/eLife.86022

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-phospho-GluR1 (Ser831) antibody, clone N453 (Rabbit polyclonal) , Millipore , Cat. # 04–823 , WB (1:1000).

Techniques: Transfection, Construct, Plasmid Preparation, Activity Assay, Expressing, Software

Journal: Molecular Brain

Article Title: DREAM (Downstream Regulatory Element Antagonist Modulator) contributes to synaptic depression and contextual fear memory

doi: 10.1186/1756-6606-3-3

Figure Lengend Snippet: Normal AMPA receptor function and expression inTgDREAM mice . (A) Input-output relationship for AMPA receptor-mediated EPSCs in wild-type (filled circles, n = 7) and TgDREAM mice (open circles, n = 7). There is no significant difference between the two groups. The sample traces are shown in the right panel. (B) Representative western blot (left) and quantified data (right) for expression levels of GluR1 and GluR2&3 subunits in hippocampus from wild-type and TgDREAM mice. Data were normalized to expression level of wild-type mice (n = 4 for each group). (C) Representative western blot (left) and quantified data (right) for phosphorylation of AMPA GluR1 receptor at ser831 and 845 sites in hippocampus from wild-type and TgDREAM mice. Data were normalized to expression level of wild-type mice (n = 4 for each group).

Article Snippet: Membranes were probed with 1:3000 dilution of anti-GluR1 (Upstate, NY), anti-GluR2&3 (Chemicon, CA), 1:2000 dilution of anti-PSD-95 (Chemicon, CA), 1:1000 dilution of anti-phospho-GluR1 Ser845 (Upstate, NY) or anti-phospho-GluR1 Ser831 (Upstate, NY), and 1:1000 dilution of anti-NR1 (Upstate, NY) anti-NR2A (Chemicon), or anti-NR2B (Chemicon) antibodies.

Techniques: Expressing, Western Blot

Journal: MedComm

Article Title: Decreased synapse‐associated proteins are associated with the onset of epileptic memory impairment in endothelial CDK5 ‐deficient mice

doi: 10.1002/mco2.128

Figure Lengend Snippet: Synapse‐related proteins decreased in hippocampus of Cdh5‐CreERT2;CDK5 f/f mice. (A and B) Representative p‐Synapsin1 (green) and PSD95 (red) staining in hippocampus of mice (A, counterstained with DAPI [blue], top right, scale bar: 20 μm; bottom, scale bar: 50 μm) and quantification of normalized p‐Synapsin1 and PSD95 intensity (B) ( **p < 0.01, * **p < 0.001, Student's t ‐test). (C and D) Representative immunoblots of synapse structure‐related proteins p‐Synapisn1/Synapsin1 (C) and PSD95 (D), and quantitative analyses by densitometry ( **p < 0.01, ns: not significant, Student's t ‐test). (E–G) Representative immunoblots of synapse receptors NMDARs (NR1 and NR2B) (F and G) and GluR1/phospho‐GluR1 (p‐GluR1) (E), and quantitative analyses by densitometry ( *p < 0.05, **p < 0.01, ns: not significant, Student's t ‐test, NR2B, n = 5; NR1, GluR1/p‐GluR1). All error bars indicate the mean value ± SEM

Article Snippet: The primary antibodies were listed as follows: phospho‐Thr286‐CaMKII (1:3000, Fukunaga et al., 1988), CaMKII (1:3000, Fukunaga et al., 1988), anti‐phospho‐Ser603‐Synapsin1 (1:1000, Millipore, AB5583), anti‐Synapsin1 (1:1000, Fukunaga et al., 1988), anti‐PSD95 (1:5000, Thermo, MA1‐045), anti‐NMDAR1 (1:1000, Abcam, ab109182), anti‐NMDAR2B (1:1000, Alomone, AGC‐003), anti‐phospho‐Ser831‐GluR1 (1:1000, Millipore, 04‐823), anti‐GluR1 (1:1000, Santa Cruz, SC‐7608), and GAPDH (1:5000, Cell Signaling Technology, Cat# 2118).

Techniques: Staining, Western Blot

Journal: Neuroscience

Article Title: LOSS OF ESTROGEN-RELATED RECEPTOR ALPHA DISRUPTS VENTRAL-STRIATAL SYNAPTIC FUNCTION IN FEMALE MICE

doi: 10.1016/j.neuroscience.2016.04.054

Figure Lengend Snippet: Increased levels of phosphorylation of GluR1 at Ser831 and Ser845 in nucleus accumbens of female Esrra-null mice. (A, B) Representative Western blot images of phosphorylated and total GluR1 in NAc (top) and densitometric analysis of phospho- to total GluR1 ratio in female WT and Esrra-null mice (bottom). Data presented as mean ± S.E.M. *p < 0.05 indicates significant differences between the groups (Mann–Whitney U test).

Article Snippet: Primary antibodies were used as follows: anti-phospho- GluR1 Ser831antibody (04–823, 1:5000, Millipore, Billerica, MA, USA), antiphospho-GluR1 Ser845 antibody (AB5849, 1:5000, Millipore) and anti-GluR1 antibody (04–855, 1:1000, Millipore), anti-beta-actin antibody (#4970, Cell Signaling, Danvers, MA, USA).

Techniques: Phospho-proteomics, Western Blot, MANN-WHITNEY